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Electrochemical method determines indinavir levels in pharmaceuticals and serum
An electrochemical method precisely determines indinavir levels in pharmaceuticals and serum.
"Indinavir sulfate is an inhibitor of the human immunodeficiency virus (HIV) protease. The aim of this study was to determine indinavir levels in serum and pharmaceuticals, by means of electrochemical methods using the hanging mercury drop electrode (HMDE)," investigators in Turkey report.
According to B. Dogan and colleagues at Ankara University, "Indinavir exhibited irreversible cathodic waves over the pH range 2.00-12.00 in different supporting electrolytes.
"The current-concentration plot was rectilinear over the range from 8x10-7 M to 8x10-6 M with a correlation coefficient of 0.996 for differential pulse voltammetry (DPV) and 8x10-7 M to 1x10-5 M with correlation of 0.999 M for osteryoung square ware voltammetry (OSWV) in Britton-Robinson buffer at pH 10.00.
"The wave was characterized as being irreversible and diffusion-controlled," researchers said.
Dogan concluded, "The proposed methods were fully validated and successfully applied to the determination of indinavir in capsules and spiked human serum samples with good recoveries.
"The repeatability and reproducibility of the methods as well as precision and accuracy (such as supporting electrolyte, serum samples) were determined. No electroactive interferences from the endogenous substances were found in serum samples."